Study on the influence of mycelium morphology of Maoyuan Streptomycetes on TG enzyme synthesis

Abstract

The aim of this study was to investigate the effect of mycelial morphology on the synthesis of glutamine transaminase (TGase) produced by Streptomyces entadiengensis. Through experiments, we found that MgCl2 stress can significantly increase the production of TG enzymes, and has an important effect on the morphology of bacteria. In this study, we first determined the optimal addition concentration of MgCl2. In addition, MgCl2 stress led to the advance of mycelium germination and spore formation, indicating that the bacteria may activate secondary metabolic regulators earlier under salt stress and promote the synthesis of TG enzymes. We also studied the effects of kaolin on the morphology and TG enzyme synthesis of Streptomyces pentadiengensis. The results showed that kaolin microparticles had the greatest effect on mycelium morphology and significantly improved TG enzyme activity, indicating that the enzyme production capacity of the strain could be effectively enhanced by regulating mycelium morphology. This study provides a new strategy for increasing the yield of TG enzymes, which is of great significance for in-depth understanding of the regulatory mechanism of microbial metabolic performance, and provides a theoretical basis for efficient production of TG enzymes in industrial production. In the future, we will further explore the specific mechanisms of kaolin influence on mycelium morphology and enzyme activity, and apply these strategies to the metabolic regulation of other microbial systems.