The effect of ploidy on recombinant expression of brewing yeast

Abstract

This study investigated the effect of different ploidy on recombinant expression in S. cerevisiae. By comparing haploid, diploid, and polyploid S. cerevisiae differences in terms of recombinant protein expression levels, expression stability, and genetic stability, we found that ploidy has a significant effect on recombinant expression in S. cerevisiae. The experimental results show that polyploid S. cerevisiae usually have higher expression levels in recombinant proteins than haploid and diploid expression proteins, probably due to polyploids containing more genomes and transcription machines, resulting in improved transcription and translation efficiency. Moreover, polyploid S. cerevisiae also showed higher expression stability, in terms that the recombinant protein expression remained relatively stable during serial passages. Therefore, for recombinant expression using S. cerevisiae, the appropriate ploidy needs to be selected according to the specific application requirements. Polyploid S. cerevisiae may be a better option for applications requiring high expression and stability; haploid or diploid S. cerevisiae may be more appropriate. This study provides an important understanding of the impact of ploidy on recombination expression in S. cerevisiae and provides new ideas and methods for optimizing the recombinant expression system in S. cerevisiae.