Phylogenetic analysis of mitochondrial genomes of Gracilaria and Gracilariopsis

Abstract

In this study, complete genome sequences of Gracilaria chouae and Gracilariopsis lemaneiformis were obtained by GenBank database. The mitochondrial genomes of Gracilaria chouae and Gracilariopsis lemaneiformis were compared and analyzed by Geneious, Mauve, tRNAscan-SE and other software. The total length of mitochondrial genome of Gracilaria chouae and Gracilariopsis lemaneiformis was 25,829bp and 25,883bp, respectively. Gracilaria chouae ncoded 50 genes, including 24 protein coding genes, 2 rRNAs, 23 tRNAs and orf148. Gracilariopsis lemaneiformis encoded 48 genes. It included 24 protein-coding genes, 2 rRNAs, 20 tRNAs, and 2 ORFs (orf60, orf143). The proportions of A, T, C and G were similar, and the content of G+C was respectively 28.8% and 27.5%. The lengths of the two RRNA genes are different, the types and numbers of the 24 protein-coding genes are the same, and the lengths of most of the protein-coding genes (17) are completely the same. atp8, nad5, nad2, sdhC, rpl16, rpl20 and secY are different in length. trnH gene is rearranged in mitochondrial genomes of Gracilaria chouae and Gracilariopsis lemaneiformis. The relevant parameters of mitochondrial genome codon are different between them. T The ENC values of mitochondrial genome of Gracilaria chouae and Gracilariopsis lemaneiformis were 50.07 and 48.85, respectively, both of which were greater than 35, indicating that the codon use of Gracilaria chouae and Gracilariopsis lemaneiformis were weak. The mean values of CBI and RSCU of Gracilaria chouae and Gracilariopsis lemaneiformis were different.