Study on preparation technology of alorulose enzyme fixed resin material

Abstract

This study focused on the biosynthesis of D-allulose enzyme, the enzyme immobilization technology and its application in industrial production. Through screening and expression of D-allulose-3-epimerase (DPE enzyme) with high efficiency and immobilization technology, the production efficiency and stability of D-allulose-3-epimerase have been successfully improved. The optimized immobilized DPE enzyme showed better temperature and pH stability and reusability, which provided a research direction for the industrial production of D-allulose. In terms of research methods, a variety of immobilization techniques such as adsorption, cross-linking, embedding and carrier binding were used in this study, and the immobilization conditions were optimized through systematic experiments. The optimal reaction conditions of the immobilized DPE enzyme in the production of D-allulose were also investigated, which provided an important reference for its application in practical production. In this study, the recombinant strain with high expression of DPE enzyme was screened, the optimal immobilization vector and conditions were determined, and the process parameters of D-allulose production were optimized. These results not only provide technical support for the industrial production of D-allulose, but also provide a new idea for the application of enzyme immobilization technology in the field of biotransformation. This study also proved that the immobilized enzyme technology had significant advantages in improving the production efficiency of D-allulose, which laid a solid foundation for the industrial production and wide application of D-allulose. D-allulose 3-epimerase has entered the domestic market, and the improvement in conversion rate, yield and production cost reduction has positive significance for the development of the industry.